Fig. 1.
MIP triggers larval settlement behavior in Platynereis. (A) Angular histograms of the displacement vectors of swimming tracks for larvae treated with DMSO (control) and larvae treated with the indicated peptide [n > 100 larvae (55–60 hpf) each]. (B) Percentage of time in which cilia are closed in DMSO-treated larvae (control) vs. larvae exposed to MIP7 [n > 10 larvae (48–50 hpf) each]. (C) Percentage of 1-, 2-, 5-, and 6-dpf larvae that showed sustained substrate contact after 2 min exposure to DMSO (control), 20 μM MIP7 (for 1 dpf and 2 dpf), or 50 μM MIP7 (for 5 dpf and 6 dpf; n = 4 repetitions with >30 larvae). (D) Percentage of larvae that showed sustained substrate contact after 90 min exposure to DMSO (control), AKYFLamide (FLa), and MIP7 [n = 10 repetitions with >30 larvae (50 hpf) each]. In A, the P values of an χ2 test comparing the number of upward and downward swimming larvae are indicated as *P < 0.05 and ***P < 0.001. Data in B–D are shown as mean ± SEM. P values of an unpaired t test are indicated as *P < 0.05, **P < 0.01, and ***P < 0.001, with “ns” indicating P > 0.05.