Fig. 4.
Molecular specification of Platynereis noncephalic photoreceptors and presence of r-opsin orthologs in the zebrafish neuromasts. (A–I) Codetection of Platynereis r-opsin (red arrowheads) and indicated transcripts (purple arrowheads) by double in situ hybridization. Coexpression is indicated by black arrows. (A–C) Expression in the adult ventral trunk. (D–G) Expression in 8-d tail regenerates (trunk and parapodia). (H and I) Expression in adult parapodia. (J–L) Expression of opn4x-2 and opn4m-2 in the zebrafish neuromast. (J) Live image of neuromast cells (green arrowheads) in the brn3c::egfp strain 6 d after fertilization; (K and L) corresponding labeling of neuromast cells (purple arrowheads) with the opn4x-2 and opn4m-2 riboprobes. (A–G) Ventral views, anterior to the left; (H and I) frontal views, dorsal to the top. (J–L) Lateral views, anterior to the left. See text for details.